Table of Contents
- Schema quick view
- International schema information
- Selection lists
- English
- Amp_Status entry codes
- HIGHSD entry codes
- Language-independent schema details
- Language-specific schema details
- English
- Schema SAIDs
Name: qRT-PCR
Description:
Classification: RDF207
Author: Chang Ge
Author Email: gec@mcmaster.ca
ICT Group: Cell Cultured Meat
Schema package SAID: EC0_5f6_6dg4JYkB8r-qPl10daGoN0pOEiIbz5xbUvpx
Download Schema
Schema quick view
| Attribute | Label | Description |
| Well | | Identifies the specific well on the qPCR plate |
| Well_Position | | Coordinates of the well |
| Sample_Name | | Name or ID of the biological or experimental sample |
| Target_Name | | Target Gene |
| Reporter | | Fluorescent dye used to detect amplification |
| CT | | Threshold cycle where fluorescence exceeds background |
| Ct_Mean | | Average CT value from technical replicates |
| Ct_SD | | Standard deviation of CT values among replicates |
| Ct_Threshold | | Fluorescence threshold used to calculate CT |
| Baseline_Start | | Start cycle for baseline signal calculation |
| Baseline_End | | End cycle for baseline signal calculation |
| Amp_Status | | Indicates whether amplification was detected |
| Cq_Conf | | Confidence in quantification cycle (Cq) value |
| HIGHSD | | High standard deviation among technical replicates for a given sample, suggesting inconsistent amplification |
| Tm1 | | Melting temperature of the first PCR product peak |
| Language | Name | Description |
| English | qRT-PCR | |
Selection lists
English
Amp_Status entry codes
| Entry code | Label |
| Amp | Amplified |
| No Amp | Not amplified |
HIGHSD entry codes
| Entry code | Label |
| Y | Yes |
| N | No |
Language-independent schema details
| Attribute | Sensitive | Unit | Type | Character encoding | Format rule |
| Well | false | | Numeric | | |
| Well_Position | false | | Text | | ^[A-Z]*$ |
| Sample_Name | false | | Text | | |
| Target_Name | false | | Text | | |
| Reporter | false | | Text | | |
| CT | false | | Numeric | | |
| Ct_Mean | false | | Numeric | | |
| Ct_SD | false | | Numeric | | |
| Ct_Threshold | false | | Numeric | | |
| Baseline_Start | false | | Numeric | | |
| Baseline_End | false | | Numeric | | |
| Amp_Status | false | | Text | | |
| Cq_Conf | false | | Numeric | | |
| HIGHSD | false | | Text | | |
| Tm1 | false | Celsius | Numeric | | |
Language-specific schema details
English
| Attribute | Label | Description | List |
| Well | | Identifies the specific well on the qPCR plate | Not a list |
| Well_Position | | Coordinates of the well | Not a list |
| Sample_Name | | Name or ID of the biological or experimental sample | Not a list |
| Target_Name | | Target Gene | Not a list |
| Reporter | | Fluorescent dye used to detect amplification | Not a list |
| CT | | Threshold cycle where fluorescence exceeds background | Not a list |
| Ct_Mean | | Average CT value from technical replicates | Not a list |
| Ct_SD | | Standard deviation of CT values among replicates | Not a list |
| Ct_Threshold | | Fluorescence threshold used to calculate CT | Not a list |
| Baseline_Start | | Start cycle for baseline signal calculation | Not a list |
| Baseline_End | | End cycle for baseline signal calculation | Not a list |
| Amp_Status | | Indicates whether amplification was detected | Amplified, Not amplified |
| Cq_Conf | | Confidence in quantification cycle (Cq) value | Not a list |
| HIGHSD | | High standard deviation among technical replicates for a given sample, suggesting inconsistent amplification | Yes, No |
| Tm1 | | Melting temperature of the first PCR product peak | Not a list |
Schema SAIDs
Capture base: EBroLoNV7McCLVmkJg_rxH2EbZS84Paapit_j_lm3UrS
Bundle: EJi38MgR5eN0HalQuMGKg0s7U1j5DrPexRRXApZlUUK1
Package: EC0_5f6_6dg4JYkB8r-qPl10daGoN0pOEiIbz5xbUvpx
| Layer | SAID | Type |
| entry (eng) | EFot7BDdwZwOVg0ef6LC_eG0i5gH-7gO4pQANjHnu7DF | spec/overlays/entry/1.1 |
| entry_code | EOh9P24N5Meuq22qYOFG2XOSORKt3wYdFCcdVMaPS4Hw | spec/overlays/entry_code/1.1 |
| format | EADAJRHFd4k8kzzjqEE0ie4hCGovN1HVT71JhnWzwEeV | spec/overlays/format/1.1 |
| information (eng) | EP87foRjGB26wfqqJv9LsWCVQVamrKNzu8SAKYUKaiWR | spec/overlays/information/1.1 |
| meta (eng) | EEImLq8eMQ5Tb9rs4UGqzEARut78vxNXYhRekQGroe_v | spec/overlays/meta/1.1 |
| unit | EPJdogWMuzK4Ipfsmyo6KGfwHc08lIY0u8Shn4lepTxq | spec/overlays/unit/1.1 |
| ordering | EEvNJw-g-ryaWI6Mv_hPztTh1a-EkEyNeXclwx9gBK6C | community/overlays/adc/ordering/1.1 |
Date created: 2025-05-02 10:56:32